Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy

S. Ohnishi, M. Hara, T. Furuno, T. Okada, H. Sasabe

研究成果: Article査読

27 被引用数 (Scopus)

抄録

The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin molecule anchored inhomogeneously in two dimensions. These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin viewed down from a 4-fold axis structure reported previously (S. H. Banyard, D. K. Stammers, and P. M. Harrison, 1978. Nature (Lond.). 271:282–284). It has been achieved by introducing a "self-screening effect" of the surface charges of the AFM sample (S. Ohnishi, M. Hara, T. Furuno, and H. Sasabe. 1992. Biophys. J. 63:1425–1431) and the specially sharpened stylus of AFM cantilever.

本文言語English
ページ(範囲)573-577
ページ数5
ジャーナルBiophysical Journal
65
2
DOI
出版ステータスPublished - 1993
外部発表はい

ASJC Scopus subject areas

  • 生物理学

フィンガープリント

「Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル