抄録
Decarboxylation of malonic acid is a well-known process in the biosynthesis of long-chain fatty acids starting from acetic acid. If this kind of enzymes exhibits enantioselectivity for asubstituted malonates, it will be useful as a novel method for preparing optically active carboxylic acids. Indeed malonyl-CoA decarboxylase from uropygial gland is enantioselective to the substrate and the product. Racemate of methylmalonyl-CoA (1) was incubated with the above decarboxylase in 3H2O. (S)-Enantiomer was smoothly decarboxylated to result in 2-(3H)-propionyl-CoA (2), while (R)-isomer of the substrate remained intact (Equation 21.1). The absolute configuration of α-carbon of resulting 2 was revealed to be (R). Thus, the decarboxylase distinguishes the chirality of 1 and gives enantiomerically pure (R)-isomer by retention of configuration [1].
本文言語 | English |
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ホスト出版物のタイトル | Biocatalysis in the Pharmaceutical and Biotechnology Industries |
出版社 | CRC Press |
ページ | 547-561 |
ページ数 | 15 |
ISBN(電子版) | 9781420019377 |
ISBN(印刷版) | 9780849337321 |
出版ステータス | Published - 2006 1月 1 |
ASJC Scopus subject areas
- 生化学、遺伝学、分子生物学(全般)
- 化学 (全般)
- 化学工学(全般)
- 医学(全般)
- 薬理学、毒性学および薬学(全般)