Distinct gene expression-defined classes of gastrointestinal stromal tumor

Umio Yamaguchi, Robert Nakayama, Kazufumi Honda, Hitoshi Ichikawa, Tadashi Hasegawa, Miki Shitashige, Masaya Ono, Ayako Shoji, Tomohiro Sakuma, Hideya Kuwabara, Yasuhiro Shimada, Mitsuru Sasako, Tadakazu Shimoda, Akira Kawai, Setsuo Hirohashi, Tesshi Yamada

研究成果: Article

94 引用 (Scopus)

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Purpose: The majority of gastrointestinal stromal tumors (GIST) can be cured by surgery alone, but relapse occurs in 20% to 40% of cases. GISTs are considered to invariably arise through gain of function KIT or PDGFA mutation of the interstitial cells of Cajal (ICC). However, the genetic basis of the malignant progression of GISTs are poorly understood. Patients and Methods: The expression levels of 54,613 probe sets in 32 surgical samples of untreated GISTs of the stomach and small intestine were analyzed with oligonucleotide microarrays. The representative GeneChip data were validated by real-time reverse transcriptase polymerase chain reaction and immunohistochemistry. Results: Unbiased hierarchical clustering consistently separated the 32 cases of GIST into two major classes according to tumor site. The two major classes were further separated into novel subclasses, which were significantly correlated with various pathological prognostic parameters, the frequency of metastasis (P < .05), and clinical outcome. Immunohistochemical analysis of 152 independent patients with gastric GISTs revealed that the expression of dipeptidyl peptidase IV (T-cell activation antigen CD26) protein was significantly associated with poorer overall and disease-free survival (P < .00001). Conclusion: CD26 appears to be a reliable biomarker of malignant GISTs of the stomach. The postoperative recurrence rate of CD26-negative cases was as low as 2.0% (two of 102). Therefore, postoperative follow-up of such patients might be made less intensive. CD26 may play an important role in the malignant progression of gastric GISTs and serve as a therapeutic target.

元の言語English
ページ(範囲)4100-4108
ページ数9
ジャーナルJournal of Clinical Oncology
26
発行部数25
DOI
出版物ステータスPublished - 2008 9 18
外部発表Yes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Yamaguchi, U., Nakayama, R., Honda, K., Ichikawa, H., Hasegawa, T., Shitashige, M., Ono, M., Shoji, A., Sakuma, T., Kuwabara, H., Shimada, Y., Sasako, M., Shimoda, T., Kawai, A., Hirohashi, S., & Yamada, T. (2008). Distinct gene expression-defined classes of gastrointestinal stromal tumor. Journal of Clinical Oncology, 26(25), 4100-4108. https://doi.org/10.1200/JCO.2007.14.2331