Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation

Shunsuke Kimura, Masami Yamashita, Megumi Yamakami-Kimura, Yusuke Sato, Atsushi Yamagata, Yoshihiro Kobashigawa, Fuyuhiko Inagaki, Takako Amada, Koji Hase, Toshihiko Iwanaga, Hiroshi Ohno, Shuya Fukai

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.

本文言語English
論文番号33548
ジャーナルScientific reports
6
DOI
出版ステータスPublished - 2016 9 15
外部発表はい

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Distinct Roles for the N-and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル