Distinct roles of ephrin-B2 forward and EphB4 reverse signaling in endothelial cells

Koichi Hamada, Yuichi Oike, Yasuhiro Ito, Hiromitsu Maekawa, Keishi Miyata, Taizo Shimomura, Toshio Suda

研究成果: Article査読

79 被引用数 (Scopus)


Objective - The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively, and bidirectional signals mediated by both proteins play an important role in vascular development. However, how such bidirectional signals are required for cell-cell adhesion or repulsion remains unclear. Methods and Results - Using a cell line and sorted primary endothelial cells, we show that ephrin-B2 forward signaling through the EphB4 receptor inhibits cell adhesion, whereas EphB4 reverse signaling by the transmembrane ephrin-B2 ligand does not. Cell migration is also inhibited on immobilized ephrin-B2-Fc but not on EphB4-Fc protein. Conclusions - Ephrin-B2 forward signaling and EphB4 reverse signaling differentially affect cell adhesion and migration between arterial and venous endothelial cells.

ジャーナルArteriosclerosis, Thrombosis, and Vascular Biology
出版ステータスPublished - 2003 2月 1

ASJC Scopus subject areas

  • 循環器および心血管医学


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