Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function

Yoshiko Masuda, Aya Sasak, Hiroshi Shibuya, Naoto Ueno, Kyoji Ikeda, Ken Watanabe

研究成果: Article

87 引用 (Scopus)

抄録

Dlx5, a member of the Dlx family of homeodomain proteins, plays a critical role in bone development and fracture healing. To understand the molecular mechanism underlying the transcriptional regulation by Dlx5, we performed yeast two-hybrid screening and isolated a novel protein, Dlxin-1, that binds Dlx5 and regulates its transcriptional function. Dlxin-1 cDNA encodes a 775-amino acid protein that has a partial homology with necdin at the C terminus and 25 repeats of hexapeptides (WQXPXX) in the middle region. Dlxin-1 mRNA is expressed in various adult tissues, but not the spleen, and also in osteoblastic and chondrogenic cell lines. During embryogenesis, a strong signal for Dlxin-1 mRNA was found in cell layers surrounding cartilaginous elements in bone rudiment during digit formation. Dlxin-1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers in vivo. Transfection and reporter gene assays indicate that Dlxin-1 activates the transcriptional function of Dlx5. Therefore, Dlxin-1 may act as a regulator of the function of Dlx family members in bone formation.

元の言語English
ページ(範囲)5331-5338
ページ数8
ジャーナルJournal of Biological Chemistry
276
発行部数7
DOI
出版物ステータスPublished - 2001 2 16
外部発表Yes

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Bone
Messenger RNA
Fracture Healing
Bone Development
Bone Fractures
Reporter Genes
Cells
Osteogenesis
Embryonic Development
Transfection
Homeodomain Proteins
Proteins
Spleen
Complementary DNA
Yeasts
Amino Acids
Bone and Bones
Cell Line
Yeast
Assays

ASJC Scopus subject areas

  • Biochemistry

これを引用

Masuda, Y., Sasak, A., Shibuya, H., Ueno, N., Ikeda, K., & Watanabe, K. (2001). Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function. Journal of Biological Chemistry, 276(7), 5331-5338. https://doi.org/10.1074/jbc.M008590200

Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function. / Masuda, Yoshiko; Sasak, Aya; Shibuya, Hiroshi; Ueno, Naoto; Ikeda, Kyoji; Watanabe, Ken.

:: Journal of Biological Chemistry, 巻 276, 番号 7, 16.02.2001, p. 5331-5338.

研究成果: Article

Masuda, Y, Sasak, A, Shibuya, H, Ueno, N, Ikeda, K & Watanabe, K 2001, 'Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function', Journal of Biological Chemistry, 巻. 276, 番号 7, pp. 5331-5338. https://doi.org/10.1074/jbc.M008590200
Masuda, Yoshiko ; Sasak, Aya ; Shibuya, Hiroshi ; Ueno, Naoto ; Ikeda, Kyoji ; Watanabe, Ken. / Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function. :: Journal of Biological Chemistry. 2001 ; 巻 276, 番号 7. pp. 5331-5338.
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