TY - JOUR
T1 - DN-cadherin is required for spatial arrangement of nerve terminals and ultrastructural organization of synapses
AU - Iwai, Youichi
AU - Hirota, Yuki
AU - Ozaki, Koichi
AU - Okano, Hideyuki
AU - Takeichi, Masatoshi
AU - Uemura, Tadashi
N1 - Funding Information:
We thank Professor S. Tsukita for providing us the opportunity to conduct electron microscopic analysis in his laboratory and we thank the members of his laboratory for advice and encouragement. We are also grateful to T. Nagao and K. Tokunaga-Furukubo for providing us a device and a protocol to section adult heads; N. Uchida, K. Sawamoto, K. Ito, Y. Hiromi, C. Hama, and E. Suzuki for advice for light and/or electronmicroscopic analyses; K. Keino-Masu for comments on the manuscript; T. K. Hensch, Y. Yoshihara, and N. Mataga for providing Y.I. with opportunities to use their computers; and S. C. Fujita, H. Bellen, S. Benzer, and the Bloomington Stock Center for reagents. Finally, we acknowledge I. A. Meinertzhagen for a number of valuable reprints. This work was supported by a grant from the program Grants-in-Aid for Creative Fundamental Research of the Ministry of Education, Science, and Culture of Japan to M.T.; by grants from the Ministry of Education, Science, and Culture of Japan and from the Human Frontier Science Program to H.O.; and by Toray Science Foundation and a grant of Core Research for Evolutional Science and Technology to T.U.
PY - 2002
Y1 - 2002
N2 - We studied roles of DN-cadherin, the Drosophila major neuronal cadherin, in neuronal connections in the visual system. In DN-cadherin mutants, axon terminals of a large subset of photoreceptor cells reached and associated with their target interneurons, but their characteristic spatial arrangement was disrupted as synaptogenesis proceeded. Although synapses were formed at contact sites between the axon terminals and target neurons, underlycytoplasmic structures were not fully specialized at both pre- and postsynaptic terminals and synaptic vesicles appeared to accumulate at the presynapses. These results suggest that the cadherin adhesion system is required for interaction between pre- and postsynaptic terminals and for generation of the mature synaptic structures.
AB - We studied roles of DN-cadherin, the Drosophila major neuronal cadherin, in neuronal connections in the visual system. In DN-cadherin mutants, axon terminals of a large subset of photoreceptor cells reached and associated with their target interneurons, but their characteristic spatial arrangement was disrupted as synaptogenesis proceeded. Although synapses were formed at contact sites between the axon terminals and target neurons, underlycytoplasmic structures were not fully specialized at both pre- and postsynaptic terminals and synaptic vesicles appeared to accumulate at the presynapses. These results suggest that the cadherin adhesion system is required for interaction between pre- and postsynaptic terminals and for generation of the mature synaptic structures.
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U2 - 10.1006/mcne.2001.1081
DO - 10.1006/mcne.2001.1081
M3 - Article
C2 - 11906210
AN - SCOPUS:0036214528
SN - 1044-7431
VL - 19
SP - 375
EP - 388
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
IS - 3
ER -