抄録
A microdevice is developed for DNA-barcode directed capture of single cells on an array of pH-sensitive microelectrodes for metabolic analysis. Cells are modified with membrane-bound single-stranded DNA, and specific single-cell capture is directed by the complementary strand bound in the sensor area of the iridium oxide pH microelectrodes within a microfluidic channel. This bifunctional microelectrode array is demonstrated for the pH monitoring and differentiation of primary T cells and Jurkat T lymphoma cells. Single Jurkat cells exhibited an extracellular acidification rate of 11 milli-pH min -1, while primary T cells exhibited only 2 milli-pH min-1. This system can be used to capture non-adherent cells specifically and to discriminate between visually similar healthy and cancerous cells in a heterogeneous ensemble based on their altered metabolic properties.
本文言語 | English |
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ページ(範囲) | 2010-2015 |
ページ数 | 6 |
ジャーナル | Lab on a Chip |
巻 | 9 |
号 | 14 |
DOI | |
出版ステータス | Published - 2009 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオエンジニアリング
- 生化学
- 化学 (全般)
- 生体医工学