@article{8a6ff02172c04991b4b2f351e3bc3c87,
title = "DNA-carrying latex particles for DNA diagnosis 2. Distinction of normal and point mutant DNA using S1 nuclease",
abstract = "In our previous paper, we reported a DNA diagnosis system in which fully complementary DNA was selectively hybridized with a DNA immobilized on latex particles but one-point mutant DNA was not. The system, however, included a few disadvantages. To overcome them, a single-strand DNA cleaving enzyme (S1 nuclease) was used in this study. S1 nuclease cleaved the unhybridized and loosely hybridized DNA's on the particles and enabled the determination of the fully complementary hybrid. This method saved us a troublesome labeling of the samples.",
keywords = "DNA diagnosis, Microsphere, Miss-matched DNA, Point-mutation, S1 nuclease",
author = "M. Hatakeyama and K. Nakamura and S. Iwato and H. Handa and K. Fujimoto and H. Kawaguchi",
note = "Funding Information: This work was supportedb y the Grant-in-Aid for ScientificR esearcho n Priority Areas, {"}New Polymersa nd Their Nano-OrganizedS ystems{"} (no. 277/08246246)f rom the ministry of Education, Science, Sports and Culture, the ResearchG rant from Keio University,a nd the SasakawaS cientific ResearchG rant from the Japan ScienceS ociety.T he authorsa cknowledge themg ratefully.",
year = "1998",
month = feb,
day = "15",
doi = "10.1016/S0927-7765(97)00058-1",
language = "English",
volume = "10",
pages = "171--178",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",
number = "3",
}