DNA cytofluorometric and nuclear morphometric analyses of lung adenocarcinoma

Hisao Asamura, Takashi Nakajima, Kiyoshi Mukai, Masayuki Noguchi, Yukio Shimosato

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The nuclear DNA content of tumor cells in imprint smears prepared from 72 surgically resected adenocarcinomas of the lung was determined prospectively by means of cytofluorometry, and its relationship to pathologic stage, degree of histologic differentiation, and nuclear atypia represented by the mean nuclear area (MNA) and standard deviation of the nuclear area (SDNA) was studied. The mean nuclear DNA content (MNDC) of poorly differentiated adenocarcinomas was significantly larger than that of well‐differentiated adenocarcinomas both in Stages I + II and III + IV (P < 0.001, each), and the MNDC of Stages III + IV was significantly larger than that of I + II in poorly differentiated adenocarcinomas (P < 0.01). There was a tendency for histogram Patterns III and IV, in which more aneuploid cells were present than in I and II, to be more common and for the incidence of the aneuploid stem cell line to be higher in less differentiated and more advanced adenocarcinomas, although the differences were not significant statistically. Furthermore, there were significant positive correlations between MNDC and MNA (P < 0.01) and between MNDC and SDNA (P < 0.05). The averages of the MNA according to the histogram pattern increased significantly in the increasing order of Patterns I to IV. These results indicate that the nuclear DNA content increases in less differentiated and more advanced adenocarcinomas, and that the nuclear atypia is reflected in abnormal nuclear DNA content (DNA aneuploidy).

本文言語English
ページ(範囲)1657-1664
ページ数8
ジャーナルCancer
64
8
DOI
出版ステータスPublished - 1989 10月 15
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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