DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity

Keiko Esashika, Toshiharu Saiki

    研究成果: Article査読

    7 被引用数 (Scopus)

    抄録

    The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.

    本文言語English
    ページ(範囲)182-189
    ページ数8
    ジャーナルBioconjugate Chemistry
    29
    1
    DOI
    出版ステータスPublished - 2018 1 17

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering
    • Pharmacology
    • Pharmaceutical Science
    • Organic Chemistry

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