DOCK2 mediates T cell receptor-induced activation of Rac2 and IL-2 transcription

Hiroshi Nishihara, Masae Maeda, Masumi Tsuda, Yoshinori Makino, Hirofumi Sawa, Kazuo Nagashima, Shinya Tanaka

研究成果: Article査読

21 被引用数 (Scopus)

抄録

DOCK2, a CDM family protein exclusively found in hematopoietic cells, has been shown to play a role in lymphocyte migration by the regulation of actin cytoskeleton. Although DOCK2 has been shown to induce the activation of Rac1, the regulatory mechanism of Rac2, which is a hematopoietic cell-specific small GTPase, is still unknown. In this study, we examined the role of DOCK2 in the activation of Rac2 in hematopoietic cells. DOCK2 was found to associate with the ζ subunit of the CD3 complex of T cell receptors in Jurkat cells and to activate forced expressed Rac2 in 293T cells. In addition, the stable expression of DOCK2 in Jurkat cells exhibited the elevated activity of endogenous Rac2. Furthermore, the transcriptional activity of interleukin-2 (IL-2) was enhanced in DOCK2-expressing Jurkat cells and the dominant negative form of Rac2 suppressed its elevated IL-2 promoter activity. These results suggest that DOCK2 mediates TCR-dependent activation of Rac2, leading to the regulation of IL-2 promoter activity in T cells.

本文言語English
ページ(範囲)716-720
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
296
3
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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