Down-regulation of EGFR prolonged cell growth of glioma but did not increase the sensitivity to temozolomide

Nobuharu Inaba, Kouki Fujioka, Hidetsugu Saito, Masaki Kimura, Keiichi Ikeda, Yuriko Inoue, Sho Ishizawa, Yoshinobu Manome

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Background: Malignant glioma is an invasive disease of the central nervous system. One of the factors that regulate growth of these tumors is expression of epidermal growth factor receptor (EGFR) in the cells. This study investigated the effects of down-regulation of EGFR on cell proliferation, cell cycle and cytotoxicity to antineoplastic agent. Materials and Methods: A short hairpin RNA transcription vector targeting EGFR was transfected into KNS42 cells. Growth curve, cell cycle and sensitivity to temozolomide of the cells were assessed. Results: Transfection inhibited EGFR expression by 50.5%. It prolonged cell doubling time by 25.7%. However, it did not meaningfully alter the cell cycle populations nor increase sensitivity to temozolomide. Conclusion: Suppressing expression of EGFR inhibited cell proliferation. However, unlike PTEN expression or ROCK1 down-regulation, it did not alter the cell cycle or increase sensitivity to temozolomide.

本文言語English
ページ(範囲)3253-3257
ページ数5
ジャーナルAnticancer research
31
10
出版ステータスPublished - 2011 10

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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