Downmodulation of IL-8 receptors, type A and type B, on human lung neutrophils in vivo

Kenzo Soejima, Seitaro Fujishima, Hidetoshi Nakamura, Yasuhiro Waki, Morio Nakamura, Hiroaki Matsubara, Sadatomo Tasaka, Koichi Sayama, Akitoshi Ishizaka, Minoru Kanazawa

研究成果: Article査読

13 被引用数 (Scopus)

抄録

We examined the expression of interleukin (IL)-8 receptors (Rs), type A (IL-8-RA) and type B (IL-8-RB), on peripheral blood and bronchoalveolar lavage (BAL) fluid neutrophils; we also examined IL-8 and other chemoattractants in the epithelial lining fluid (ELF) of patients with chronic lower respiratory tract infection (CLI) to elucidate the in vivo regulation of IL-8Rs. Neutrophils were stained with monoclonal antibodies specific for IL-8-RA and IL-8-RB. We detected higher levels of IL-8 (81.6 ± 25.4 ng/ml, mean ± SE), leukotriene (LT) B4, and IL-1β in the ELF of the CLI patients than in their serum (P < 0.05). The expression of IL-8Rs on BAL neutrophils was significantly lower than that on peripheral blood neutrophils (P < 0.01 for both). In vitro analysis showed that low-level IL-8 (50 ng/ml) alone did not affect IL-8R expression but that it was downregulated by high- level IL-8 (500 ng/ml) alone and by low-level IL-8 in combination with LTB4 or IL-1β. Staurosporine reduced the downmodulation by low-level IL-8 plus LTB4 or IL-1β but not by high-level IL-8 alone. We speculate that pulmonary IL-8-RA and IL-8-RB may have been downmodulated by the combined effect of local chemoattractants through, in part, a protein kinase C-dependent mechanism.

本文言語English
ページ(範囲)L618-L625
ジャーナルAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
273
3 17-3
DOI
出版ステータスPublished - 1997 9月
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 呼吸器内科
  • 生理学(医学)
  • 細胞生物学

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