Dual temperature-and pH-responsive fluorescence molecular probe for cellular imaging utilizing a PNIPAAm-fluorescein copolymer

Hiroki Kobayashi, Mayumi Nishikawa, Chikako Sakamoto, Tadashi Nishio, Hideko Kanazawa, Teruo Okano

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The thermally controlling fluorescence intensity of a molecular probe for cellular imaging has been investigated. A reversible temperature-induced phase transition of N-isopropylacrylamide/fluorescein copolymers [poly(NIPAAm-co-FL)] was used as a molecular switch to control the fluorescence intensity of the imaging probe. The copolymer displayed environmentally sensitive fluorescence properties, in which the fluorescence intensity changed with the response to both the temperature and the pH. Utilizing these features, we monitored the thermal-aggregation process of BSA by the fluorescence resonance energy transfer (FRET) method. Additionally, the cellular uptake in RAW264.7 cells of poly(NIPAAm-co-FL) conjugated with lipid was studied using a confocal laser scanning microscope. 2009

本文言語English
ページ(範囲)1043-1047
ページ数5
ジャーナルanalytical sciences
25
8
DOI
出版ステータスPublished - 2009 8月
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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