Dynamic control of cystic fibrosis transmembrane conductance regulator Cl-/HCO3- selectivity by external Cl -

Nikolay Shcheynikov, Kil Hwan Kim, Kyung Mi Kim, Michael R. Dorwart, Shigeru B.H. Ko, Hidemi Goto, Satoru Naruse, Philip J. Thomas, Shmuel Muallem

研究成果: Article

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HCO3- secretion is a vital activity in cystic fibrosis transmembrane conductance regulator (CFTR)-expressing epithelia. However, the role of CFTR in this activity is not well understood. Simultaneous measurements of membrane potential and pHi and/or current in CFTR-expressing Xenopus oocytes revealed dynamic control of CFTR Cl -/HCO3- permeability ratio, which is regulated by external Cl- (Clo-). Thus, reducing external Cl- from 110 to 0-10 mM resulted in the expected increase in membrane potential, but with no corresponding OH- or HCO 3- influx. Approximately 3-4 min after reducing Cl o- to 0 mM, an abrupt switch in membrane potential occurs that coincided with an increased rates of OH- and HCO 3- influx. The switch in membrane permeability to OH -/HCO3- can also be recorded as a leftward shift in the reversal potential. Furthermore, an increased rate of OH - influx in response to elevating pHo to 9.0 was observed only after the switch in membrane potential. The time to switch increased to 11 min at Clo- of 5 mM. Conversely, re-addition of external Cl- after the switch in membrane potential did not stop HCO 3- influx, which continued for about 3.9 min after Cl - addition. Importantly, addition of external Cl- to cells incubated in Cl--free medium never resulted in HCO 3- efflux. Voltage and current clamp experiments showed that the delayed HCO3- transport is electrogenic. These results indicate that CFTR exists in two conformations, a Cl- only and a Cl- and OH-/HCO3- permeable state. The switch between the states is controlled by external Cl-. Accordingly, a different tryptic pattern of CFTR was found upon digestion in Cl--containing and Cl--free media. The physiological significance of these finding is discussed in the context of HCO 3- secretion by tissues such as the pancreas and salivary glands.

元の言語English
ページ(範囲)21857-21865
ページ数9
ジャーナルJournal of Biological Chemistry
279
発行部数21
DOI
出版物ステータスPublished - 2004 5 21

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント Dynamic control of cystic fibrosis transmembrane conductance regulator Cl<sup>-</sup>/HCO<sub>3</sub><sup>-</sup> selectivity by external Cl <sup>-</sup>' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Shcheynikov, N., Kim, K. H., Kim, K. M., Dorwart, M. R., Ko, S. B. H., Goto, H., Naruse, S., Thomas, P. J., & Muallem, S. (2004). Dynamic control of cystic fibrosis transmembrane conductance regulator Cl-/HCO3- selectivity by external Cl -. Journal of Biological Chemistry, 279(21), 21857-21865. https://doi.org/10.1074/jbc.M313323200