Dynamic Flow Velocity Mapping from Fluorescent Dye Transit Times in the Brain Surface Microcirculation of Anesthetized Rats and Mice

Ryo Hoshikawa, Hiroshi Kawaguchi, Hiroyuki Takuwa, Yoko Ikoma, Yutaka Tomita, Miyuki Unekawa, Norihiro Suzuki, Iwao Kanno, Kazuto Masamoto

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Objective: This study aimed to develop a new method for mapping blood flow velocity based on the spatial evolution of fluorescent dye transit times captured with CLSFM in the cerebral microcirculation of anesthetized rodents. Methods: The animals were anesthetized with isoflurane, and a small amount of fluorescent dye was intravenously injected to label blood plasma. The CLSFM was conducted through a closed cranial window to capture propagation of the dye in the cortical vessels. The transit time of the dye over a certain distance in a single vessel was determined with automated image analyses, and average flow velocity was mapped in each vessel. Results: The average flow velocity measured in the rat pial artery and vein was 4.4 ± 1.2 and 2.4 ± 0.5 mm/sec, respectively. A similar range of flow velocity to those of the rats was observed in the mice; 4.9 ± 1.4 and 2.0 ± 0.9 mm/sec, respectively, although the vessel diameter in the mice was about half of that in the rats. Conclusions: Flow velocity in the cerebral microcirculation can be mapped based on fluorescent dye transit time measurements with conventional CLSFM in experimental animals.

本文言語English
ページ(範囲)416-425
ページ数10
ジャーナルMicrocirculation
23
6
DOI
出版ステータスPublished - 2016 8月 1

ASJC Scopus subject areas

  • 生理学
  • 分子生物学
  • 循環器および心血管医学
  • 生理学(医学)

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