Dynamin isoforms decode action potential firing for synaptic vesicle recycling

Shota Tanifuji, Megumi Funakoshi-Tago, Fumihito Uedas, Tadashi Kasaharas, Sumiko Mochida

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Presynaptic nerve terminals must maintain stable neurotransmission via synaptic vesicle membrane recycling despite encountering wide fluctuations in the number and frequency of incoming action potentials (APs). However, the molecular mechanism linking variation in neuronal activity to vesicle trafficking is unknown. Here, we combined genetic knockdown and direct physiological measurements of synaptic transmission from paired neurons to show that three isoforms of dynamin, an essential endocytic protein, work individually to match vesicle reuse pathways, having distinct rate and time constants with physiological AP frequencies. Dynamin 3 resupplied the readily releasable pool with slow kinetics independently of the AP frequency but acted quickly, within 20 ms of the incoming AP. Under high-frequency firing, dynamin 1 regulated recycling to the readily releasable pool with fast kinetics in a slower time window of greater than 50 ms. Dynamin 2 displayed a hybrid response between the other isoforms. Collectively, our findings show how dynamin isoforms select appropriate vesicle reuse pathways associated with specific neuronal firing patterns.

本文言語English
ページ(範囲)19050-19059
ページ数10
ジャーナルJournal of Biological Chemistry
288
26
DOI
出版ステータスPublished - 2013 6 28

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Dynamin isoforms decode action potential firing for synaptic vesicle recycling」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル