@inproceedings{948eb9beeed64f4594a931ad32970b6a,
title = "E-CELL: Towards integrative modeling of cellular processes",
keywords = "E-CELL, in silico biology, Mycoplasma genitalium, simulation, systems biology, virtual cell",
author = "Masaru Tomita",
note = "Funding Information: A major bottleneck in cell modeling is lack of quantitative data, such as kinetic parameters, dissociation constants, steady state concentration, and flux rates. Metabolome Group of our institute is developing methodologies for mass-production of those quantitative metabolic data. We analyze metabolic flux distributions (MFDs) with different conditions such as dissolved oxygen (DO) concentration, pH, temperature, and media composition. We also label certain substrates with U-13C or 1-13C, and measure isotope distribution of intercellular metabolites using NMR and GC-MS. For high- throughput measurement of metabolites, we have developed a novel analytical device based on capillary electrophoresis and mass spectrograph, which can measure hundreds of metabolites at the same time [4]. Based on data collected by those means, we are developing a quantitative and dynamic model of E. coli energy metabolism as a part of Cell Modeling Project funded by New Energy and Industrial Technology Development Organization (NEDO) of the Ministry of Economy, Trade and Industry of Japan [5]; 32. Jahrestagung der Gesellschaft fur Informatik, Informatik bewegt, INFORMATIK 2002 - 32nd Annual Conference of the German Informatics Society, Informatics Moves, INFORMATIK 2002 ; Conference date: 30-09-2002 Through 03-10-2002",
year = "2002",
language = "English",
series = "Lecture Notes in Informatics (LNI), Proceedings - Series of the Gesellschaft fur Informatik (GI)",
publisher = "Gesellschaft fur Informatik (GI)",
pages = "45--46",
editor = "Schubert, {Sigrid E.} and Bernd Reusch and Norbert Jesse",
booktitle = "32. Jahrestagung der Gesellschaft fur Informatik, Informatik bewegt, INFORMATIK 2002",
address = "Germany",
}