ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow

Azusa Shimizu; Wei H. Goh; Rahul B. Karyappa; Michinao Hashiomoto; Hiroaki Onoe

doi:10.1109/MEMS46641.2020.9056188

ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow

Azusa Shimizu, Wei H. Goh, Rahul B. Karyappa, Michinao Hashiomoto, Hiroaki Onoe

機械工学科

研究成果: Conference contribution

抄録

We present an extracellular matrix(ECM)-based microfluidic device that creates a spatial chemical gradient by interstitial flow. Cells located on surfaces of ECM, such as vascular and skin tissues, are influenced by external factors, including interstitial flows, but few methods have been available to recapitulate such conditions. To address this gap, we developed a microfluidic device that can expose cells cultured on the surface of ECM to a continuous concentration gradient created by the interstitial flow. With a 3D printed water-soluble sacrificial mold, we readily fabricated ECM-based microfluidic devices embedding micromixers and a gradient generator. ECM mimicked the permeability of in vivo environments. Our device will serve as a platform to study cellular phenomena occurring on the surface of ECM, such as vascularization and migration of cancer cells.

本文言語	English
ホスト出版物のタイトル	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
出版社	Institute of Electrical and Electronics Engineers Inc.
ページ	122-124
ページ数	3
ISBN（電子版）	9781728135809
DOI	https://doi.org/10.1109/MEMS46641.2020.9056188
出版ステータス	Published - 2020 1月
イベント	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada 継続期間: 2020 1月 18 → 2020 1月 22

出版物シリーズ

名前	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
巻	2020-January
ISSN（印刷版）	1084-6999

Conference

Conference	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
国/地域	Canada
City	Vancouver
Period	20/1/18 → 20/1/22

ASJC Scopus subject areas

電子材料、光学材料、および磁性材料
凝縮系物理学
機械工学
電子工学および電気工学

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

文献へのアクセス

10.1109/MEMS46641.2020.9056188

他のファイルとリンク

引用スタイル

Shimizu, A., Goh, W. H., Karyappa, R. B., Hashiomoto, M., & Onoe, H. (2020). ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 (pp. 122-124). [9056188] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS46641.2020.9056188

ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow. / Shimizu, Azusa; Goh, Wei H.; Karyappa, Rahul B. その他.
33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. p. 122-124 9056188 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January).

研究成果: Conference contribution

Shimizu, A, Goh, WH, Karyappa, RB, Hashiomoto, M & Onoe, H 2020, ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow. in 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020., 9056188, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2020-January, Institute of Electrical and Electronics Engineers Inc., pp. 122-124, 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020, Vancouver, Canada, 20/1/18. https://doi.org/10.1109/MEMS46641.2020.9056188

Shimizu A, Goh WH, Karyappa RB, Hashiomoto M, Onoe H. ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc. 2020. p. 122-124. 9056188. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS46641.2020.9056188

Shimizu, Azusa ; Goh, Wei H. ; Karyappa, Rahul B. その他. / ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow. 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. pp. 122-124 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "We present an extracellular matrix(ECM)-based microfluidic device that creates a spatial chemical gradient by interstitial flow. Cells located on surfaces of ECM, such as vascular and skin tissues, are influenced by external factors, including interstitial flows, but few methods have been available to recapitulate such conditions. To address this gap, we developed a microfluidic device that can expose cells cultured on the surface of ECM to a continuous concentration gradient created by the interstitial flow. With a 3D printed water-soluble sacrificial mold, we readily fabricated ECM-based microfluidic devices embedding micromixers and a gradient generator. ECM mimicked the permeability of in vivo environments. Our device will serve as a platform to study cellular phenomena occurring on the surface of ECM, such as vascularization and migration of cancer cells.",

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note = "Funding Information: This work was partly supported by Grant-in Aid for Scientific Research (B) (19H04440) from Japan Society for the Promotion of Science (JSPS), Japan, and the research grant from Digital Manufacturing and Design Centre (DManD) at Singapore University of Technology and Design (SUTD) (RGDM1620503). Publisher Copyright: {\textcopyright} 2020 IEEE.; 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 ; Conference date: 18-01-2020 Through 22-01-2020",

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N1 - Funding Information: This work was partly supported by Grant-in Aid for Scientific Research (B) (19H04440) from Japan Society for the Promotion of Science (JSPS), Japan, and the research grant from Digital Manufacturing and Design Centre (DManD) at Singapore University of Technology and Design (SUTD) (RGDM1620503). Publisher Copyright: © 2020 IEEE.

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N2 - We present an extracellular matrix(ECM)-based microfluidic device that creates a spatial chemical gradient by interstitial flow. Cells located on surfaces of ECM, such as vascular and skin tissues, are influenced by external factors, including interstitial flows, but few methods have been available to recapitulate such conditions. To address this gap, we developed a microfluidic device that can expose cells cultured on the surface of ECM to a continuous concentration gradient created by the interstitial flow. With a 3D printed water-soluble sacrificial mold, we readily fabricated ECM-based microfluidic devices embedding micromixers and a gradient generator. ECM mimicked the permeability of in vivo environments. Our device will serve as a platform to study cellular phenomena occurring on the surface of ECM, such as vascularization and migration of cancer cells.

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ECM-Based Gradient Generator for Tunable Surface Environment by Interstitial Flow

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出版物シリーズ

Conference

ASJC Scopus subject areas

UN SDG

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