ECM-based microfluidic gradient generator for tunable surface environment by interstitial flow

Azusa Shimizu, Wei Huang Goh, Shun Itai, Rahul Karyappa, Michinao Hashimoto, Hiroaki Onoe

研究成果: Article査読

3 被引用数 (Scopus)


We present an extracellular matrix (ECM)-based gradient generator that provides a culture surface with continuous chemical concentration gradients created by interstitial flow. The gelatin-based microchannels harboring gradient generators and in-channel micromixers were rapidly fabricated by sacrificial molding of a 3D-printed water-soluble sacrificial mold. When fluorescent dye solutions were introduced into the channel, the micromixers enhanced mixing of two solutions joined at the junction. Moreover, the concentration gradients generated in the channel diffused to the culture surface of the device through the interstitial space facilitated by the porous nature of the ECM. To check the functionality of the gradient generator for investigating cellular responses to chemical factors, we demonstrated that human umbilical vein endothelial cells cultured on the surface shrunk in response to the concentration gradient of histamine generated by interstitial flow from the microchannel. We believe that our device could be useful for the basic biological study of the cellular response to chemical stimuli and for the in vitro platform in drug testing.

出版ステータスPublished - 2020 7月 1

ASJC Scopus subject areas

  • 生体医工学
  • 材料科学(全般)
  • 凝縮系物理学
  • 流体および伝熱
  • コロイド化学および表面化学


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