Ectopic expression of blood type antigens in inflamed mucosa with higher incidence of FUT2 secretor status in colonic Crohn's disease

Jun Miyoshi, Tomoharu Yajima, Susumu Okamoto, Katsuyoshi Matsuoka, Nagamu Inoue, Tadakazu Hisamatsu, Katsuyoshi Shimamura, Atsushi Nakazawa, Takanori Kanai, Haruhiko Ogata, Yasushi Iwao, Makio Mukai, Toshifumi Hibi

研究成果: Article

19 引用 (Scopus)

抄録

Background Host-intestinal microbial interaction plays an important role in the pathogenesis of inflammatory bowel diseases (IBDs). The surface molecules of the intestinal epithelium act as receptors for bacterial adhesion and regulate the intestinal bacteria. Some known receptors are the mucosal blood type antigens, which are regulated by the fucosyltransferase2 (FUT2) gene, and individuals who express these antigens in the gastrointestinal tract are called secretors. Recent research has revealed that the FUT2 gene is associated with Crohn's disease (CD) in western populations. Methods To clarify the contribution of mucosal blood type antigens in IBD, we determined the incidence of five previously reported single-nucleotide polymorphisms of the FUT2 gene in Japanese patients. We also used immunohistochemistry to investigate the antigen expression in mucosal specimens from IBD patients and animal models. Results Genetic analysis revealed that all of the patients with colonic CD were secretors, whereas the incidence of secretors was 80, 80, 67, and 80%, respectively, for the control, ileocolonic CD, ileal CD, and ulcerative colitis groups (P = 0.036). Abnormal expression of blood type antigens was observed only in colonic CD. Interleukin- 10 -/- mice, but not dextran sulfate sodium colitis mice, had enhanced colonic expression of blood type antigens, and the expression of these antigens preceded the development of colitis in the interleukin-10 -/- mice. Conclusions FUT2 secretor status was associated with colonic-type CD. This finding, taken together with the immunohistochemistry data, suggests that the abnormal expression of blood type antigens in the colon may be a unique and essential factor for colonic CD.

元の言語English
ページ(範囲)1056-1063
ページ数8
ジャーナルJournal of Gastroenterology
46
発行部数9
DOI
出版物ステータスPublished - 2011 9

Fingerprint

Colonic Diseases
Crohn Disease
Mucous Membrane
Antigens
Incidence
Inflammatory Bowel Diseases
Colitis
Interleukin-10
Ileal Diseases
Immunohistochemistry
Microbial Interactions
Genes
Bacterial Adhesion
Dextran Sulfate
Ectopic Gene Expression
Intestinal Mucosa
Ulcerative Colitis
Single Nucleotide Polymorphism
Gastrointestinal Tract
Colon

ASJC Scopus subject areas

  • Gastroenterology

これを引用

Ectopic expression of blood type antigens in inflamed mucosa with higher incidence of FUT2 secretor status in colonic Crohn's disease. / Miyoshi, Jun; Yajima, Tomoharu; Okamoto, Susumu; Matsuoka, Katsuyoshi; Inoue, Nagamu; Hisamatsu, Tadakazu; Shimamura, Katsuyoshi; Nakazawa, Atsushi; Kanai, Takanori; Ogata, Haruhiko; Iwao, Yasushi; Mukai, Makio; Hibi, Toshifumi.

:: Journal of Gastroenterology, 巻 46, 番号 9, 09.2011, p. 1056-1063.

研究成果: Article

Miyoshi, Jun ; Yajima, Tomoharu ; Okamoto, Susumu ; Matsuoka, Katsuyoshi ; Inoue, Nagamu ; Hisamatsu, Tadakazu ; Shimamura, Katsuyoshi ; Nakazawa, Atsushi ; Kanai, Takanori ; Ogata, Haruhiko ; Iwao, Yasushi ; Mukai, Makio ; Hibi, Toshifumi. / Ectopic expression of blood type antigens in inflamed mucosa with higher incidence of FUT2 secretor status in colonic Crohn's disease. :: Journal of Gastroenterology. 2011 ; 巻 46, 番号 9. pp. 1056-1063.
@article{01fa7ec9636647f49fce0741b6b97b1a,
title = "Ectopic expression of blood type antigens in inflamed mucosa with higher incidence of FUT2 secretor status in colonic Crohn's disease",
abstract = "Background Host-intestinal microbial interaction plays an important role in the pathogenesis of inflammatory bowel diseases (IBDs). The surface molecules of the intestinal epithelium act as receptors for bacterial adhesion and regulate the intestinal bacteria. Some known receptors are the mucosal blood type antigens, which are regulated by the fucosyltransferase2 (FUT2) gene, and individuals who express these antigens in the gastrointestinal tract are called secretors. Recent research has revealed that the FUT2 gene is associated with Crohn's disease (CD) in western populations. Methods To clarify the contribution of mucosal blood type antigens in IBD, we determined the incidence of five previously reported single-nucleotide polymorphisms of the FUT2 gene in Japanese patients. We also used immunohistochemistry to investigate the antigen expression in mucosal specimens from IBD patients and animal models. Results Genetic analysis revealed that all of the patients with colonic CD were secretors, whereas the incidence of secretors was 80, 80, 67, and 80{\%}, respectively, for the control, ileocolonic CD, ileal CD, and ulcerative colitis groups (P = 0.036). Abnormal expression of blood type antigens was observed only in colonic CD. Interleukin- 10 -/- mice, but not dextran sulfate sodium colitis mice, had enhanced colonic expression of blood type antigens, and the expression of these antigens preceded the development of colitis in the interleukin-10 -/- mice. Conclusions FUT2 secretor status was associated with colonic-type CD. This finding, taken together with the immunohistochemistry data, suggests that the abnormal expression of blood type antigens in the colon may be a unique and essential factor for colonic CD.",
keywords = "Blood type antigen, Colonic Crohn's disease, FUT2",
author = "Jun Miyoshi and Tomoharu Yajima and Susumu Okamoto and Katsuyoshi Matsuoka and Nagamu Inoue and Tadakazu Hisamatsu and Katsuyoshi Shimamura and Atsushi Nakazawa and Takanori Kanai and Haruhiko Ogata and Yasushi Iwao and Makio Mukai and Toshifumi Hibi",
year = "2011",
month = "9",
doi = "10.1007/s00535-011-0425-7",
language = "English",
volume = "46",
pages = "1056--1063",
journal = "Journal of Gastroenterology",
issn = "0944-1174",
publisher = "Springer Japan",
number = "9",

}

TY - JOUR

T1 - Ectopic expression of blood type antigens in inflamed mucosa with higher incidence of FUT2 secretor status in colonic Crohn's disease

AU - Miyoshi, Jun

AU - Yajima, Tomoharu

AU - Okamoto, Susumu

AU - Matsuoka, Katsuyoshi

AU - Inoue, Nagamu

AU - Hisamatsu, Tadakazu

AU - Shimamura, Katsuyoshi

AU - Nakazawa, Atsushi

AU - Kanai, Takanori

AU - Ogata, Haruhiko

AU - Iwao, Yasushi

AU - Mukai, Makio

AU - Hibi, Toshifumi

PY - 2011/9

Y1 - 2011/9

N2 - Background Host-intestinal microbial interaction plays an important role in the pathogenesis of inflammatory bowel diseases (IBDs). The surface molecules of the intestinal epithelium act as receptors for bacterial adhesion and regulate the intestinal bacteria. Some known receptors are the mucosal blood type antigens, which are regulated by the fucosyltransferase2 (FUT2) gene, and individuals who express these antigens in the gastrointestinal tract are called secretors. Recent research has revealed that the FUT2 gene is associated with Crohn's disease (CD) in western populations. Methods To clarify the contribution of mucosal blood type antigens in IBD, we determined the incidence of five previously reported single-nucleotide polymorphisms of the FUT2 gene in Japanese patients. We also used immunohistochemistry to investigate the antigen expression in mucosal specimens from IBD patients and animal models. Results Genetic analysis revealed that all of the patients with colonic CD were secretors, whereas the incidence of secretors was 80, 80, 67, and 80%, respectively, for the control, ileocolonic CD, ileal CD, and ulcerative colitis groups (P = 0.036). Abnormal expression of blood type antigens was observed only in colonic CD. Interleukin- 10 -/- mice, but not dextran sulfate sodium colitis mice, had enhanced colonic expression of blood type antigens, and the expression of these antigens preceded the development of colitis in the interleukin-10 -/- mice. Conclusions FUT2 secretor status was associated with colonic-type CD. This finding, taken together with the immunohistochemistry data, suggests that the abnormal expression of blood type antigens in the colon may be a unique and essential factor for colonic CD.

AB - Background Host-intestinal microbial interaction plays an important role in the pathogenesis of inflammatory bowel diseases (IBDs). The surface molecules of the intestinal epithelium act as receptors for bacterial adhesion and regulate the intestinal bacteria. Some known receptors are the mucosal blood type antigens, which are regulated by the fucosyltransferase2 (FUT2) gene, and individuals who express these antigens in the gastrointestinal tract are called secretors. Recent research has revealed that the FUT2 gene is associated with Crohn's disease (CD) in western populations. Methods To clarify the contribution of mucosal blood type antigens in IBD, we determined the incidence of five previously reported single-nucleotide polymorphisms of the FUT2 gene in Japanese patients. We also used immunohistochemistry to investigate the antigen expression in mucosal specimens from IBD patients and animal models. Results Genetic analysis revealed that all of the patients with colonic CD were secretors, whereas the incidence of secretors was 80, 80, 67, and 80%, respectively, for the control, ileocolonic CD, ileal CD, and ulcerative colitis groups (P = 0.036). Abnormal expression of blood type antigens was observed only in colonic CD. Interleukin- 10 -/- mice, but not dextran sulfate sodium colitis mice, had enhanced colonic expression of blood type antigens, and the expression of these antigens preceded the development of colitis in the interleukin-10 -/- mice. Conclusions FUT2 secretor status was associated with colonic-type CD. This finding, taken together with the immunohistochemistry data, suggests that the abnormal expression of blood type antigens in the colon may be a unique and essential factor for colonic CD.

KW - Blood type antigen

KW - Colonic Crohn's disease

KW - FUT2

UR - http://www.scopus.com/inward/record.url?scp=80755128479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80755128479&partnerID=8YFLogxK

U2 - 10.1007/s00535-011-0425-7

DO - 10.1007/s00535-011-0425-7

M3 - Article

C2 - 21725903

AN - SCOPUS:80755128479

VL - 46

SP - 1056

EP - 1063

JO - Journal of Gastroenterology

JF - Journal of Gastroenterology

SN - 0944-1174

IS - 9

ER -