Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells: A 31P nuclear magnetic resonance study

Keiichi Torimitsu, Yoshiaki Yazaki, Kinuyo Nagasuka, Eiji Ohta, Makoto Sakata

研究成果: Article

34 引用 (Scopus)

抄録

The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the 31P nuclear magnetic resonance (NMR) method. The 31P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Pi and vacuolar Pi. The cytoplasmic and vacuolar pHs could be determined by comparing the Pi chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H+-ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H+-ATPase appears to have an important part in the regulation of the intracellular pH.

元の言語English
ページ(範囲)1403-1409
ページ数7
ジャーナルPlant and Cell Physiology
25
発行部数8
出版物ステータスPublished - 1984 12

Fingerprint

Mung Bean
Meristem
Nuclear Magnetic Resonance
ATP Synthase
mung beans
Pi
root tips
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Roots
Cell
Chemical shift
Titration
Inhibitor
Breakdown
inhibitor
Strictly
cells

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

これを引用

Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells : A 31P nuclear magnetic resonance study. / Torimitsu, Keiichi; Yazaki, Yoshiaki; Nagasuka, Kinuyo; Ohta, Eiji; Sakata, Makoto.

:: Plant and Cell Physiology, 巻 25, 番号 8, 12.1984, p. 1403-1409.

研究成果: Article

Torimitsu, K, Yazaki, Y, Nagasuka, K, Ohta, E & Sakata, M 1984, 'Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells: A 31P nuclear magnetic resonance study', Plant and Cell Physiology, 巻. 25, 番号 8, pp. 1403-1409.
Torimitsu, Keiichi ; Yazaki, Yoshiaki ; Nagasuka, Kinuyo ; Ohta, Eiji ; Sakata, Makoto. / Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells : A 31P nuclear magnetic resonance study. :: Plant and Cell Physiology. 1984 ; 巻 25, 番号 8. pp. 1403-1409.
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