Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells: A 31P nuclear magnetic resonance study

Keiichi Torimitsu, Yoshiaki Yazaki, Kinuyo Nagasuka, Eiji Ohta, Makoto Sakata

研究成果: Article

34 引用 (Scopus)

抜粋

The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the 31P nuclear magnetic resonance (NMR) method. The 31P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Pi and vacuolar Pi. The cytoplasmic and vacuolar pHs could be determined by comparing the Pi chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H+-ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H+-ATPase appears to have an important part in the regulation of the intracellular pH.

元の言語English
ページ(範囲)1403-1409
ページ数7
ジャーナルPlant and Cell Physiology
25
発行部数8
DOI
出版物ステータスPublished - 1984 12
外部発表Yes

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

フィンガープリント Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells: A <sup>31</sup>P nuclear magnetic resonance study' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用