Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts

Matsuhiko Hayashi, Yasuyoshi Yamaji, Masahiro Iyori, Waichi Kitajima, Takao Saruta

研究成果: Article査読

36 被引用数 (Scopus)

抄録

To examine the mechanisms which regulate the functions of the intercalated cells (ICs) in the cortical collecting duct (CCD), the effect of isoproterenol on intracellular pH (pHi) of ICs was studied with the in vitro microperfused rabbit CCD, using the single cell pHi determination technique with fluorescent dye, 2′,7′-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein. The pHi of β-IC was significantly decreased with the addition of basolateral 10-6 M isoproterenol (7.21±0.04 to 7.05±0.04), whereas α-IC did not show any change. This response of β-IC to isoproterenol was dose-dependent and completely inhibited by the β-blockers, atenolol or propranolol. The addition of forskolin or 8-Br-cAMP mimicked the effects of isoproterenol, suggesting that the activation of adenylate cyclase induced the decrease in pHi. The rate of pHi changes after the Cl- removal from the perfusate, which is considered to reflect the activity of luminal anion exchanger, was significantly higher with isoproterenol (0.032±0.009 pH unit/s) than that in the control (0.023±0.009 pH unit/s). The present studies provide direct evidence for the regulation of β-IC function by β-adrenergic receptor; and the luminal Cl-/HCO3- exchanger was considered to be stimulated by β-agonist, directly.

本文言語English
ページ(範囲)1153-1157
ページ数5
ジャーナルJournal of Clinical Investigation
87
4
DOI
出版ステータスPublished - 1991 4月
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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