Effect of masticatory stimulation on the quantity and quality of saliva and the salivary metabolomic profile

Nobuyuki Okuma, Makiko Saita, Noriyuki Hoshi, Tomoyoshi Soga, Masaru Tomita, Masahiro Sugimoto, Katsuhiko Kimoto

研究成果: Article

8 引用 (Scopus)

抄録

Background: This study characterized the changes in quality and quantity of saliva, and changes in the salivary metabolomic profile, to understand the effects of masticatory stimulation. Methods: Stimulated and unstimulated saliva samples were collected from 55 subjects and salivary hydrophilic metabolites were comprehensively quantified using capillary electrophoresis-time-of-flight mass spectrometry. Results: In total, 137 metabolites were identified and quantified. The concentrations of 44 metabolites in stimulated saliva were significantly higher than those in unstimulated saliva. Pathway analysis identified the upregulation of the urea cycle and synthesis and degradation pathways of glycine, serine, cysteine and threonine in stimulated saliva. A principal component analysis revealed that the effect of masticatory stimulation on salivary metabolomic profiles was less dependent on sample population sex, age, and smoking. The concentrations of only 1 metabolite in unstimulated saliva, and of 3 metabolites stimulated saliva, showed significant correlation with salivary secretion volume, indicating that the salivary metabolomic profile and salivary secretion volume were independent factors. Conclusions: Masticatory stimulation affected not only salivary secretion volume, but also metabolite concentration patterns. A low correlation between the secretion volume and these patterns supports the conclusion that the salivary metabolomic profile may be a new indicator to characterize masticatory stimulation.

元の言語English
記事番号e0183109
ジャーナルPLoS One
12
発行部数8
DOI
出版物ステータスPublished - 2017 8 1

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Metabolomics
metabolomics
Metabolites
saliva
Saliva
metabolites
secretion
Capillary electrophoresis
Threonine
capillary electrophoresis
Principal component analysis
Capillary Electrophoresis
Glycine
Serine
Mass spectrometry
Cysteine
Urea
Principal Component Analysis
glycine (amino acid)
threonine

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Effect of masticatory stimulation on the quantity and quality of saliva and the salivary metabolomic profile. / Okuma, Nobuyuki; Saita, Makiko; Hoshi, Noriyuki; Soga, Tomoyoshi; Tomita, Masaru; Sugimoto, Masahiro; Kimoto, Katsuhiko.

:: PLoS One, 巻 12, 番号 8, e0183109, 01.08.2017.

研究成果: Article

Okuma, Nobuyuki ; Saita, Makiko ; Hoshi, Noriyuki ; Soga, Tomoyoshi ; Tomita, Masaru ; Sugimoto, Masahiro ; Kimoto, Katsuhiko. / Effect of masticatory stimulation on the quantity and quality of saliva and the salivary metabolomic profile. :: PLoS One. 2017 ; 巻 12, 番号 8.
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