Effect of meso-Hexestrol, a Synthetic Estrogen, on S-Tubulin

Aiko Hirata, Michio Matsuhashi, Yumiko Sakakibara, Taiko Oda, Yoshihiro Sato

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We have reported that meso-hexestrol a synthetic estrogen, inhibits microtubule assembly and induces microtubule proteins into twisted ribbon structures. On the other hand, Serrano et a/. proved that S-tubulin, which lacks the C-terminal moiety of tubulin subunits, assembles into sheet structures in the absence of microtubule-associated proteins (MAPs). In the present investigation, we attempted to clarify whether meso-hexestrol could induce the ribbon structure from S-tubulin. meso-Hexestrol delayed the initiation of polymerization of S-tubulin into sheet structures in a dose-dependent manner below 50 pM. But the effect of meso-hexestrol on S-tubulin was reduced in the presence of either tau or microtubule-associated protein 2 (MAP2) in a MAPs-concentration-dependent manner. At concentrations higher than 100/im, meso-hexestrol inhibited the polymerization of S-tubulin into sheet structures, without forming ribbon structures. The present results may indicate that moso-hexestrol interacts with S-tubulin, and its interaction is affected by MAPs.

本文言語English
ページ(範囲)3419-3422
ページ数4
ジャーナルChemical and Pharmaceutical Bulletin
38
12
DOI
出版ステータスPublished - 1990 1月 1

ASJC Scopus subject areas

  • 化学 (全般)
  • 創薬

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