Effect of-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells

N. Kuzumaki, A. Suzuki, M. Narita, T. Hosoya, A. Nagasawa, S. Imai, K. Yamamizu, H. Morita, H. Nagase, Y. Okada, H. J. Okano, J. K. Yamashita, H. Okano, T. Suzuki, M. Narita

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Background: It is becoming increasingly recognised that opioids are responsible for tumour growth. However, the effects of opioids on tumour growth have been controversial.Methods:The effects of-opioid receptor (KOR) agonist on the growth of non-small cell lung cancer (NSCLC) cells were assessed by a cell proliferation assay. Western blotting was performed to ascertain the mechanism by which treatment with KOR agonist suppresses tumour growth. Results: Addition of the selective KOR agonist U50,488H to gefitinib-sensitive (HCC827) and gefitinib-resistant (H1975) NSCLC cells produced a concentration-dependent decrease in their growth. These effects were abolished by co-treatment with the selective KOR antagonist nor-BNI. Furthermore, the growth-inhibitory effect of gefitinib in HCC827 cells was further enhanced by co-treatment with U50,488H. With regard to the inhibition of tumour growth, the addition of U50, 488H to H1975 cells produced a concentration-dependent decrease in phosphorylated- glycogen synthase kinase 3Β (p-GSK3Β).Conclusion:The present results showed that stimulation of KOR reduces the growth of gefitinib-resistant NSCLC cells through the activation of GSK3Β.

本文言語English
ページ(範囲)1148-1152
ページ数5
ジャーナルBritish Journal of Cancer
106
6
DOI
出版ステータスPublished - 2012 3月 13

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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