Effect of rat CINC/gro, a member of the interleukin-8 family, on leukocytes in microcirculation of the rat mesentery

Kazuyoshi Watanabe, Makoto Suematsu, Megumi Iida, Kiyosumi Takaishi, Yoshio Iizuka, Hidekazu Suzuki, Masayuki Suzuki, Masaharu Tsuchiya, Susumu Tsurufuji

研究成果: Article査読

74 被引用数 (Scopus)

抄録

Rat cytokine-induced neutrophil chemoattractant (CINC) is a member of the IL-8 family, and its human counterpart is gro/MGSA but not IL-8. We ascertained that chemically synthesized CINC was comparable to native CINC/gro with regard to chemotactic activity for rat neutrophils and studied the effect of synthesized CINC/gro on circulating leukocytes in microvascular vessels of rat mesentery. Exposure of rat mesentery to 10-8 M authentic CINC/gro induced neutrophil adherence to and extravasation from postcapillary venules (PCVs) but not from capillaries or arterioles. CINC/gro concentrations as low as 10-10 M were effective in causing neutrophil adherence. Neutrophils adhered to thin PCVs (mean diameter, ∼25 μm) after exposure to CINC/gro for 15 min. The mean diameters of the PCV with adherence of neutrophils after exposure to CINC/gro for 30 and 60 min were 37 and 43 μm, respectively. The diameters of PCV with extravasation of neutrophils also increased in a time-dependent manner. The starting position of adherence of neutrophils was ∼25-50 μm away from the upper junction of two vessels and remained virtually unchanged during exposure to CINC/gro for 60 min. However, the distance from the start to the end of neutrophil adherence increased in a time-dependent manner. The effect of CINC/gro on adherence and extravasation of leukocytes was neutrophil specific since other leukocytes such as lymphocytes and monocytes were not identified among the adherent and extravasated leukocytes.

本文言語English
ページ(範囲)60-69
ページ数10
ジャーナルExperimental and Molecular Pathology
56
1
DOI
出版ステータスPublished - 1992 2月

ASJC Scopus subject areas

  • 病理学および法医学
  • 分子生物学
  • 臨床生化学

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