TY - JOUR
T1 - Effect of refrigeration of blood samples in lithium-heparin tubes on QuantiFERON TB Gold Plus test result
AU - Uwamino, Yoshifumi
AU - Sakai, Akiko
AU - Nishimura, Tomoyasu
AU - Noguchi, Masayo
AU - Uno, Shunsuke
AU - Fujiwara, Hiroshi
AU - Mori, Masaaki
AU - Wakui, Masatoshi
AU - Murata, Mitsuru
AU - Hasegawa, Naoki
N1 - Funding Information:
This study was funded by Kyokuto Pharmaceutical Industries, Inc. (Tokyo, Japan).
Publisher Copyright:
© 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases
PY - 2020/3
Y1 - 2020/3
N2 - The QuantiFERON TB Gold Plus (QFT-Plus) test is a newly approved interferon-gamma releasing assay test for detecting latent tuberculosis. Although blood samples for QFT test can be refrigerated for 48 h in lithium-heparin tubes according to package inserts, no published data are available on the effects of sample refrigeration on the test results. We conducted a clinical study that aimed to elucidate whether sample refrigeration for 48 h affects QFT-Plus test results. We collected 2 blood samples each from 40 participants for QFT-Plus; one sample was refrigerated before incubation for QFT-Plus assay, while the other sample was incubated soon after collection and treated as control. After comparing QFT-Plus test results of refrigerated samples and control samples, the concordance rate and kappa coefficient between them were 95% and 0.90, respectively. Thus, blood samples for QFT-Plus test can be refrigerated for 48 h in lithium-heparin tubes without influencing the test results.
AB - The QuantiFERON TB Gold Plus (QFT-Plus) test is a newly approved interferon-gamma releasing assay test for detecting latent tuberculosis. Although blood samples for QFT test can be refrigerated for 48 h in lithium-heparin tubes according to package inserts, no published data are available on the effects of sample refrigeration on the test results. We conducted a clinical study that aimed to elucidate whether sample refrigeration for 48 h affects QFT-Plus test results. We collected 2 blood samples each from 40 participants for QFT-Plus; one sample was refrigerated before incubation for QFT-Plus assay, while the other sample was incubated soon after collection and treated as control. After comparing QFT-Plus test results of refrigerated samples and control samples, the concordance rate and kappa coefficient between them were 95% and 0.90, respectively. Thus, blood samples for QFT-Plus test can be refrigerated for 48 h in lithium-heparin tubes without influencing the test results.
KW - Latent tuberculosis infection
KW - QuantiFERON TB Gold Plus
KW - Refrigeration
KW - Sample storage
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U2 - 10.1016/j.jiac.2019.10.002
DO - 10.1016/j.jiac.2019.10.002
M3 - Article
C2 - 31836288
AN - SCOPUS:85076246525
SN - 1341-321X
VL - 26
SP - 312
EP - 314
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
IS - 3
ER -
