Effect of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1) on airway epithelial cells

Osamu Narumoto, Yuichi Niikura, Satoshi Ishii, Hirofumi Morihara, Saki Okashiro, Takashi Nakahari, Takashi Nakano, Hitoshi Matsumura, Chikao Shimamoto, Yasuhiro Moriwaki, Hidemi Misawa, Naohide Yamashita, Takahide Nagase, Koichiro Kawashima, Naomi Yamashita

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Acetylcholine (ACh) exerts various anti-inflammatory effects through α7 nicotinic ACh receptors (nAChRs). We have previously shown that secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1), a positive allosteric modulator of α7 nAChR signaling, is down-regulated both in an animal model of asthma and in human epithelial cells treated with an inflammatory cytokine related to asthma. Our aim of this study was to explore the effect of SLURP-1, signal through α7 nAChR, in the pathophysiology of airway inflammation. Cytokine production was examined using human epithelial cells. Ciliary beat frequency of murine trachea was measured using a high speed camera. The IL-6 and TNF-α production by human epithelial cells was augmented by siRNA of SLURP-1 and α7 nicotinic ACh receptor. The cytokine production was also dose-dependently suppressed by human recombinant SLURP-1 (rSLURP-1). The ciliary beat frequency and amplitude of murine epithelial cells were augmented by PNU282987, a selective α7 nAChR agonist. Those findings suggested that SLURP-1 and stimulus through α7 nicotinic ACh receptors actively controlled asthmatic condition by stimulating ciliary beating and also by suppressing airway inflammation.

本文言語English
ページ(範囲)175-179
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
438
1
DOI
出版ステータスPublished - 2013 8 16

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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