Effective Cell Transfection in An Ultrasonically Levitated Droplet for Sustainable Technology

研究成果: Article査読

抄録

The levitation methodology, which enables us to operate a contactless reaction without a container, is likely to be a revolutionary technology in the fields of chemistry and biology to reduce the plastic waste in life science laboratories. Here, the authors show that plasmid DNA can be effectively transfected into animal cells in a floating droplet of culture medium levitated using ultrasonic standing waves. The data indicate that there is no significant damage to the plasmid and cells during the levitating transfection time, and the transgene expression efficiency and cellular uptake in the droplet are significantly higher than those in the conventional tube, with and without shaking. These results suggest the consolidation of the endocytic uptake pathway into macropinocytosis, indicating that ultrasonic levitation induced a change in cell characteristics. This study suggests that transfection methodology using ultrasonic levitation has the potential to advance the current experimental procedures in the field of cell engineering, in addition to presenting a revolutionary containerless reactor for sustainable technology.

本文言語English
論文番号2203576
ジャーナルAdvanced Science
9
30
DOI
出版ステータスPublished - 2022 10月 25

ASJC Scopus subject areas

  • 医学(その他)
  • 化学工学(全般)
  • 材料科学(全般)
  • 生化学、遺伝学、分子生物学(その他)
  • 工学(全般)
  • 物理学および天文学(全般)

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