TY - JOUR
T1 - Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads
AU - Mizutani, Aya
AU - Nagase, Kenichi
AU - Kikuchi, Akihiko
AU - Kanazawa, Hideko
AU - Akiyama, Yoshikatsu
AU - Kobayashi, Jun
AU - Annaka, Masahiko
AU - Okano, Teruo
N1 - Funding Information:
Present research was supported in part by the Development of New Environmental Technology using Nanotechnology Project of the National Institute of Environmental Science (NIES), commissioned from the Ministry of Environment, Japan , and Grants-in-Aid for Scientific Research (B) No. 19591568 from the Japan Society for the Promotion of Science . The authors are grateful to Dr. Norio Ueno, Tokyo Women's Medical University for English editing.
PY - 2010/8
Y1 - 2010/8
N2 - For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.
AB - For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.
KW - Atom transfer radical polymerization
KW - Peptide
KW - Poly(N-isopropylacrylamide)
KW - Poly(styrene-co-divinylbenzene)
KW - Polymer brush
KW - Thermo-responsive chromatography
KW - Thermo-responsive polymer
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U2 - 10.1016/j.jchromb.2010.06.026
DO - 10.1016/j.jchromb.2010.06.026
M3 - Article
C2 - 20634151
AN - SCOPUS:77955413070
SN - 1570-0232
VL - 878
SP - 2191
EP - 2198
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 24
ER -