Effectiveness of plasma treatment on gastric cancer cells

Koji Torii, Suguru Yamada, Kae Nakamura, Hiromasa Tanaka, Hiroaki Kajiyama, Kuniaki Tanahashi, Naoki Iwata, Mitsuro Kanda, Daisuke Kobayashi, Chie Tanaka, Tsutomu Fujii, Goro Nakayama, Masahiko Koike, Hiroyuki Sugimoto, Shuji Nomoto, Atsushi Natsume, Michitaka Fujiwara, Masaaki Mizuno, Masaru Hori, Hideyuki SayaYasuhiro Kodera

研究成果: Article査読

68 被引用数 (Scopus)

抄録

Background: Treatment of peritoneal carcinomatosis arising from gastric cancer remains a considerable challenge. In recent years, the anticancer effect of nonequilibrium atmospheric pressure plasma (NEAPP) has been reported in several cancer cell lines. Use of NEAPP may develop into a new class of anticancer therapy that augments surgery, chemotherapy, and radiotherapy. Method: Gastric cancer cells were assessed for changes in cell morphology and rate of proliferation after treatment with NEAPP-exposed medium (PAM). To explore the functional mechanism, caspase 3/7, annexin V, and uptake of reactive oxygen species (ROS) were evaluated, along with the effect of the ROS scavenger N-acetylcysteine (NAC). Results: PAM treatment for 24 h affected cell morphology, suggestive of induction of apoptosis. PAM cytotoxicity was influenced by the time of exposure to PAM, the type of cell line, and the number of cells seeded. Cells treated with PAM for 2 h demonstrated activated caspase 3/7 and an increased proportion of annexin V-positive cells compared with untreated cells. Additionally, ROS uptake was observed in PAM-treated cells, whereas NAC reduced the cytotoxicity induced by PAM presumably through reduction of ROS uptake. Furthermore, CD44 variant 9, which reportedly leads to glutathione synthesis and suppresses stress signaling of ROS, was overexpressed in PAM-resistant cells. Conclusions: PAM treatment induced apoptosis of gastric cancer cells through generation and uptake of ROS. Local administration of PAM could develop into an option to treat peritoneal carcinomatosis.

本文言語English
ページ(範囲)635-643
ページ数9
ジャーナルGastric Cancer
18
3
DOI
出版ステータスPublished - 2015 7月 27

ASJC Scopus subject areas

  • 腫瘍学
  • 消化器病学
  • 癌研究

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