Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA

Hitomi Takenaka, Naoko Takahashi, Jong Ryul Lee, Masaki Makise, Yoshihiro Yamaguchi, Tomofusa Tsuchiya, Tohru Mizushima

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.

本文言語English
ページ(範囲)652-655
ページ数4
ジャーナルBiological and Pharmaceutical Bulletin
25
5
DOI
出版ステータスPublished - 2002 5
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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