Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary

Alexei A. Sharov, Geppino Falco, Yulan Piao, Suresh Poosala, Kevin G. Becker, Alan B. Zonderman, Dan L. Longo, David Schlessinger, Minoru S.H. Ko

研究成果: Article査読

50 被引用数 (Scopus)


Background: The aging of reproductive organs is not only a major social issue, but of special interest in aging research. A long-standing view of 'immortal germ line versus mortal soma' poses an important question of whether the reproductive tissues age in similar ways to the somatic tissues. As a first step to understand this phenomenon, we examine global changes in gene expression patterns by DNA microarrays in ovaries and testes of C57BL/6 mice at 1, 6, 16, and 24 months of age. In addition, we compared a group of mice on ad libitum (AL) feeding with a group on lifespan-extending 40% calorie restriction (CR). Results: We found that gene expression changes occurred in aging gonads, but were generally different from those in somatic organs during aging. For example, only two functional categories of genes previously associated with aging in muscle, kidney, and brain were confirmed in ovary: genes associated with complement activation were upregulated, and genes associated with mitochondrial electron transport were downregulated. The bulk of the changes in gonads were mostly related to gonad-specific functions. Ovaries showed extensive gene expression changes with age, especially in the period when ovulation ceases (from 6 to 16 months), whereas testes showed only limited age-related changes. The same trend was seen for the effects of CR: CR-mediated reversal of age-associated gene expression changes, reported in somatic organs previously, was limited to a small number of genes in gonads. Instead, in both ovary and testis, CR caused small and mostly gonad-specific effects: suppression of ovulation in ovary and activation of testis-specific genes in testis. Conclusion: Overall, the results are consistent with unique modes of aging and its modification by CR in testis and ovary.

ジャーナルBMC Biology
出版ステータスPublished - 2008 6月 3

ASJC Scopus subject areas

  • バイオテクノロジー
  • 構造生物学
  • 生態、進化、行動および分類学
  • 生理学
  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 植物科学
  • 発生生物学
  • 細胞生物学


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