We have reported that (+)-, (-)- and (±)-indenestrols A and B (IA and IB respectively) inhibit the polymerization of microtubule proteins isolated from porcine brain in vitro. In this study, the effects of (+)-, (-)- and (±)-IA and IB on the relative plating efficiency, chromosome number and cellular microtubular architecture of Chinese hamster V79 cells, detected with a fluorescent anti-tubulin antibody, were investigated. The results indicated that the effect of (±)-IA was similar to that of diethylstilbestrol and that of (±)-IB was greater than that of (±)-IA. We also determined the effects of the optically active IA and IB isomers and found that the rank order of cytotoxic activity of the IA and IB series was: (-)-IA>(±)-IA>(+)-IA and (±)-IB≧(-)-IB>(+)-IB. Furthermore, we studied the intracellular disturbance of microtubule formation induced by these compounds and found that (-)-IA had by far the greatest disruptive effect.
|ジャーナル||Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis|
|出版ステータス||Published - 1993 10月|
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