Effects of bistheonellide A, an actin-polymerization inhibitor, on Chinese hamster V79 cells and on IL-8 production in PMA-stimulated HL-60 cells

Taiko Oda, Jinzhong Xu, Ayako Fujita, Masataka Mochizuki, Michio Namikoshi

研究成果: Article

4 引用 (Scopus)

抄録

Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC 50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL-60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.

元の言語English
ページ(範囲)22-27
ページ数6
ジャーナルMarine Drugs
4
発行部数1
DOI
出版物ステータスPublished - 2006 3

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HL-60 Cells
interleukin-8
Chinese hamsters
Cricetulus
Interleukin-8
polymerization
Polymerization
actin
Actins
inhibitor
sponge
production system
bioassay
ethanol
Theonella
cells
Palau
Porifera
Microtubules
Biological Assay

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Aquatic Science

これを引用

Effects of bistheonellide A, an actin-polymerization inhibitor, on Chinese hamster V79 cells and on IL-8 production in PMA-stimulated HL-60 cells. / Oda, Taiko; Xu, Jinzhong; Fujita, Ayako; Mochizuki, Masataka; Namikoshi, Michio.

:: Marine Drugs, 巻 4, 番号 1, 03.2006, p. 22-27.

研究成果: Article

Oda, Taiko ; Xu, Jinzhong ; Fujita, Ayako ; Mochizuki, Masataka ; Namikoshi, Michio. / Effects of bistheonellide A, an actin-polymerization inhibitor, on Chinese hamster V79 cells and on IL-8 production in PMA-stimulated HL-60 cells. :: Marine Drugs. 2006 ; 巻 4, 番号 1. pp. 22-27.
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abstract = "Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC 50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL-60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.",
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AU - Oda, Taiko

AU - Xu, Jinzhong

AU - Fujita, Ayako

AU - Mochizuki, Masataka

AU - Namikoshi, Michio

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AB - Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC 50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL-60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.

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