Effects of Cu, Zn-superoxide dismutase delivered by genetically modified skin fibroblasts on cold-induced skin edema in rats

Kohshi Nishiguchi, Fusao Komada, Yusuke Tanigawara, Yumiko Sakai, Seigo Iwakawa, Katsuhiko Okumura

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The effects of Cu, Zn-superoxide disumutase (SOD) delivered by genetically modified skin fibroblasts on cold-induced skin edema were studied in rats. Cold-induced skin edema, was induced on the dorsal skin following transplantation of ILSOD cells, genetically modified skin fibroblasts which release secretable SOD protein into the extracellular space. The degree of skin edema induced by cold injury was estimated by measuring the amounts of Evans' blue (EB) leaking into the injured skin following intravenously administration. The amounts of EB leakage were significantly reduced by transplantation of ILSOD cells relative to that observed following transplantation of host cells as a control. The degrees and durability of these effects of ILSOD cells were dependent on the number of cells transplanted. Also, the increases of lipid peroxidation following cold injury were significantly reduced by transplantation of ILSOD cells but not of host cells. These findings suggested that transplantation of ILSOD cells was a suitable delivery system for obtaining efficient and continuous effects of SOD. This strategy using genetically modified skin fibroblasts may also be useful as a drug delivery system for other therapeutic proteins.

本文言語English
ページ(範囲)1379-1381
ページ数3
ジャーナルBiological and Pharmaceutical Bulletin
21
12
DOI
出版ステータスPublished - 1998 1 1

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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