Effects of Cyclic Adenosine 3′,5′-Monophosphate on Chondrocyte Terminal Differentiation and Cartilage-Matrix Calcification

Akitoshi Jikko, Hiroshi Murakami, Weiqun Yan, Kazuhisa Nakashima, Yoshie Ohya, Hisashi Satakeda, Mitsuhide Noshiro, Takeshi Kawamoto, Shigeo Nakamura, Yasunori Okada, Fujio Suzuki, Yukio Kato

研究成果: Article査読

30 被引用数 (Scopus)

抄録

We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1α,25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 μM), whereas 10-to 30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocytes and cartilage-matrix calcification.

本文言語English
ページ(範囲)122-128
ページ数7
ジャーナルEndocrinology
137
1
DOI
出版ステータスPublished - 1996
外部発表はい

ASJC Scopus subject areas

  • Endocrinology

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