Effects of diethylstilbestrol and its methyl ethers on aneuploidy induction and microtubule distribution in Chinese hamster V79 cells

Yumiko Sakakibara, Izumi Saito, Ken Ichinoseki, Taiko Oda, Motohisa Kaneko, Hazime Saitô, Masahiko Kodama, Yoshihiro Sato

研究成果: Article査読

60 被引用数 (Scopus)

抄録

We previously reported that diethylstilbestrol (DES) and its derivatives inhibit the in vitro polymerization of microtubule proteins isolated from porcine brain (Sato et al., 1987). We found that the presence of the free hydroxy group of DES was indispensable for the inhibition of microtubule assembly. In the present investigation, this structure-activity relationship was confirmed by the effects of DES and its methyl ethers on chromosome number and the cellular microtubule architecture of Chinese hamster V79 cells, revealed by fluorescent anti-tubulin antibody. DES induced tetra- and octa-ploidy and DES monomethyl ether induced only tetraploidy at a much slower rate, whereas DES dimethyl ether was found to be completely inactive. Furthermore, DES was more active than its monomethyl ether in disturbing microtubule formation within cells. These results support the initial assumption that polyploidy is largely a consequence of the disturbed assembly of microtubules.

本文言語English
ページ(範囲)269-276
ページ数8
ジャーナルMutation Research Letters
263
4
DOI
出版ステータスPublished - 1991 8月

ASJC Scopus subject areas

  • 医学(全般)

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