Effects of laser parameters on propagation characteristics of laser-induced stress wave for gene transfer

Takahiro Ando, Shunichi Sato, Mitsuhiro Terakawa, Hiroshi Ashida, Minoru Obara

    研究成果: Conference contribution

    1 被引用数 (Scopus)


    Laser-based gene delivery is attractive as a new method for topical gene therapy because of the high spatial controllability of laser energy. Previously, we demonstrated that an exogenous gene can be transferred to cells both in vitro and in vivo by applying nanosecond pulsed laser-induced stress waves (LISWs) or photomechanical waves (PMWs). In this study, we investigated effects of laser parameters on the propagation characteristics of LISWs in soft tissue phantoms and depth-dependent properties of gene transfection. Temporal pressure profiles of LISWs were measured with a hydrophone, showing that with a larger laser spot diameter, LISWs can be propagated more efficiently in phantoms with keeping flat wavefront. Phantoms with various thicknesses were placed on the rat dorsal skin that had been injected with plasmid DNA coding for reporter gene, and LISWs were applied from the top of the phantom. Efficient gene expression was observed in the rat skin that had interacted with LISWs propagating through a 15-mm-thick phantom. These results would be useful to determine appropriate laser parameters for gene delivery to deep-located tissue by transcutaneous application of LISWs.

    ホスト出版物のタイトルOptical Interactions with Tissues and Cells XXI
    出版ステータスPublished - 2010 5 6
    イベントOptical Interactions with Tissues and Cells XXI - San Francisco, CA, United States
    継続期間: 2010 1 252010 1 27


    名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE


    OtherOptical Interactions with Tissues and Cells XXI
    CountryUnited States
    CitySan Francisco, CA

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Atomic and Molecular Physics, and Optics
    • Biomaterials
    • Radiology Nuclear Medicine and imaging

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