Effects of ONO-1078, a peptide leukotriene antagonist, on endotoxin- induced acute lung injury

A. Ishizaka, N. Hasegawa, F. Sakamaki, S. Tasaka, H. Nakamura, K. Kishikawa, A. Yamada, T. Obata, K. Sayama, T. Urano, M. Kanazawa

研究成果: Article査読

19 被引用数 (Scopus)

抄録

The role of lipoxygenase metabolites in the pathogenesis of endotoxin (LPS)-induced lung injury remains to be clarified. We investigated the contribution of peptide leukotrienes to LPS-induced acute lung injury using a potent antagonist, ONO-1078 (ONO). Experimental groups consisted of a saline group (n = 10), an LPS group (n = 9) injected intravenously with 2 mg E. coli LPS, an ONO group (n = 8) receiving 30 mg/kg of intraperitoneal ONO, and an LPS + ONO group (n = 6) receiving 30 mg/kg of ONO intraperitoneally 10 min before the LPS injection. The [125I]albumin lung plasma ratio, which is a parameter of acute lung injury, was significantly increased (p < 0.01) in the LPS group compared with the saline, ONO, and LPS + ONO groups. The [125I]albumin BAL fluid plasma ratio was also increased (p < 0.01) in the LPS group compared with the other groups. ONO pretreatment attenuated the LPS-induced increases in neutrophil counts in the BAL fluid. In vitro studies showed that ONO suppresses the neutrophil chemotaxis induced by LTB4, zymosan-activated serum, and FMLP. We conclude that (1) ONO-1078 attenuates LPS-induced acute lung injury; and (2) this effect appears mainly a result of its potent antagonistic actions against peptide leukotrienes and also, in part, the suppression of neutrophil chemotaxis.

本文言語English
ページ(範囲)1325-1331
ページ数7
ジャーナルAmerican journal of respiratory and critical care medicine
150
5 I
DOI
出版ステータスPublished - 1994

ASJC Scopus subject areas

  • 呼吸器内科
  • 集中医療医学

フィンガープリント

「Effects of ONO-1078, a peptide leukotriene antagonist, on endotoxin- induced acute lung injury」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル