Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs

Akiko Hozumi, Narudo Kawai, Reiko Yoshida, Yosuke Ogura, Naoyuki Ohta, Honoo Satake, Nori Satoh, Yasunori Sasakura

研究成果: Article査読

31 被引用数 (Scopus)

抄録

Transgenesis with transposons is an important technique for studying genetic functions. In the ascidian Ciona intestinalis, methods for germline transformation with the Tc1/mariner transposon Minos have been established. A system to remobilize a single Minos copy in the genome is needed to refine this transgenic technique. In this study, such an experimental system was established with a transgenic line expressing Minos transposase in eggs. In the eggs of a double transgenic animal from a cross between the egg transposase line and a transgenic line having a single Minos insertion, the transposon was transposed into new positions of the Ciona genome, thus creating new insertions. Some of the new insertions caused enhancer detection. The majority of the new insertion sites were mapped on different chromosomes from that of the transposon donor. This characteristic of Minos is in contrast to that of the Sleeping Beauty transposon, which causes frequent intrachromosomal transposition.

本文言語English
ページ(範囲)1076-1088
ページ数13
ジャーナルDevelopmental Dynamics
239
4
DOI
出版ステータスPublished - 2010 4 1

ASJC Scopus subject areas

  • 発生生物学

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