Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

Hirohito Yamazaki, Takaha Mizuguchi, Keiko Esashika, Toshiharu Saiki

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Complicated DNA molecular behaviors exist during translocation into a nanopore because their large and coiled structure needs to unwind. In this work, we investigated DNA translocation dynamics through a 200 nm pore using a fast photon counting system (FPCS). We found that the dwell time of the DNA molecules depends on the inverse of voltage (τ ∝ V-1.02) with a large constant term (∼1 ms). In other words, spherical fluorescence bead translocation involves electrophoresis as well as other additional factors. Our theoretical calculation suggested that one additional factor is electro-osmotic trapping associated with the instantaneous Brownian motion before and after translocation. Furthermore, compressed DNA molecular conformation was seen as a result of the increase of peak photon counts and the decrease of electrophoretic mobility with voltage. Our experiments showed that the polymers at the vicinity of a nanopore can be trapped and compressed, which is necessary to understand how to control the polymer translocation into a nanopore.

本文言語English
ページ(範囲)5381-5388
ページ数8
ジャーナルAnalyst
144
18
DOI
出版ステータスPublished - 2019 9 21

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

フィンガープリント 「Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル