Electro-osmotic trapping and compression of single DNA molecules while passing through a nanopore

Hirohito Yamazaki, Takaha Mizuguchi, Keiko Esashika, Toshiharu Saiki

    研究成果: Article査読

    2 被引用数 (Scopus)

    抄録

    Complicated DNA molecular behaviors exist during translocation into a nanopore because their large and coiled structure needs to unwind. In this work, we investigated DNA translocation dynamics through a 200 nm pore using a fast photon counting system (FPCS). We found that the dwell time of the DNA molecules depends on the inverse of voltage (τ ∝ V-1.02) with a large constant term (∼1 ms). In other words, spherical fluorescence bead translocation involves electrophoresis as well as other additional factors. Our theoretical calculation suggested that one additional factor is electro-osmotic trapping associated with the instantaneous Brownian motion before and after translocation. Furthermore, compressed DNA molecular conformation was seen as a result of the increase of peak photon counts and the decrease of electrophoretic mobility with voltage. Our experiments showed that the polymers at the vicinity of a nanopore can be trapped and compressed, which is necessary to understand how to control the polymer translocation into a nanopore.

    本文言語English
    ページ(範囲)5381-5388
    ページ数8
    ジャーナルAnalyst
    144
    18
    DOI
    出版ステータスPublished - 2019 9 21

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy
    • Electrochemistry

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