Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

M. Tabata, H. Yang, F. Mannan, Y. Katayama, T. Goda, A. Matsumoto, A. Seichi, K. Suzuki, Y. Miyahara

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.

本文言語English
ホスト出版物のタイトル2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1553-1556
ページ数4
ISBN(印刷版)9781479989553
DOI
出版ステータスPublished - 2015 8 5
イベント18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
継続期間: 2015 6 212015 6 25

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
国/地域United States
CityAnchorage
Period15/6/2115/6/25

ASJC Scopus subject areas

  • 器械工学
  • 電子工学および電気工学

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