Elevation of KL-6, a lung epithelial cell marker, in plasma and epithelial lining fluid in acute respiratory distress syndrome

Akitoshi Ishizaka, Tomoyuki Matsuda, Kurt H. Albertine, Hidefumi Koh, Sadatomo Tasaka, Naoki Hasegawa, Nobuoki Kohno, Toru Kotani, Hiroshi Morisaki, Junzo Takeda, Morio Nakamura, Xiaohui Fang, Thomas R. Martin, Michael A. Matthay, Satoru Hashimoto

研究成果: Article

124 引用 (Scopus)

抜粋

KL-6 is a pulmonary epithelial mucin more prominently expressed on the surface membrane of alveolar type II cells when these cells are proliferating, stimulated, and/or injured. We hypothesized that high levels of KL-6 in epithelial lining fluid and plasma would reflect the severity of lung injury in patients with acute lung injury (ALI). Epithelial lining fluid was obtained at onset (day 0) and day 1 of acute respiratory distress syndrome (ARDS)/ALI by bronchoscopic microsampling procedure in 35 patients. On day 0, KL-6 and albumin concentrations in epithelial lining fluid were significantly higher than in normal controls (P < 0.001), and the concentrations of KL-6 in epithelial lining fluid (P < 0.002) and in plasma (P < 0.0001) were higher in nonsurvivors than in survivors of ALI/ARDS. These observations were corroborated by the immunohistochemical localization of KL-6 protein expression in the lungs of nonsurvivors with ALI and KL-6 secretion from cultured human alveolar type II cells stimulated by proinflammatory cytokines. Because injury to distal lung epithelial cells, including alveolar type II cells, is important in the pathogenesis of ALI, the elevation of KL-6 concentrations in plasma and epithelial lining fluid could be valuable indicators for poor prognosis in clinical ALI.

元の言語English
ページ(範囲)L1088-L1094
ジャーナルAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
286
発行部数6 30-6
DOI
出版物ステータスPublished - 2004 6 1

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ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

これを引用

Ishizaka, A., Matsuda, T., Albertine, K. H., Koh, H., Tasaka, S., Hasegawa, N., Kohno, N., Kotani, T., Morisaki, H., Takeda, J., Nakamura, M., Fang, X., Martin, T. R., Matthay, M. A., & Hashimoto, S. (2004). Elevation of KL-6, a lung epithelial cell marker, in plasma and epithelial lining fluid in acute respiratory distress syndrome. American Journal of Physiology - Lung Cellular and Molecular Physiology, 286(6 30-6), L1088-L1094. https://doi.org/10.1152/ajplung.00420.2002