ELISA-CIEF using capillary-based microdevice: Highly-sensitive elisa based on capillary-isoelectric focusing of enzyme reaction product

Yuta Uenoyama, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun Ichi Funano, Terence G. Henares, Tatsuro Endo, Hideaki Hisamoto

研究成果: Conference contribution

抄録

Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approximately seven-fold enhancement of sensitivity and 1-2 orders of magnitude lower the detection limit were achieved.

本文言語English
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版社Chemical and Biological Microsystems Society
ページ1735-1737
ページ数3
ISBN(印刷版)9780979806452
出版ステータスPublished - 2012 1 1
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
継続期間: 2012 10 282012 11 1

出版物シリーズ

名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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