Enantioselective binding sites on bovine serum albumin to dansyl amino acids

Yoshihiro Abe, Shikie Fukui, Yuki Koshiji, Michi Kobayashi, Tomoko Shoji, Setsuro Sugata, Hideyuki Nishizawa, Hiroshi Suzuki, Kazunori Iwata

研究成果: Article査読

19 被引用数 (Scopus)

抄録

The enantioselective binding sites on bovine serum albumin were examined by HPLC using 19 racemic 5-N,N-dimethylamino-1-naphthalenesulfonyl derivatives of α-amino acids (dansyl amino acids) as chiral probes. On a bovine serum albumin bonded chiral stationary phase, seven L-forms eluted faster than their D-forms, while ten D-forms eluted before their L-forms. It was speculated that either two classes or two different binding sites exist on bovine serum albumin which can be distinguished by N-dansyl-L-proline and N-dansyl-D-norvaline. This was confirmed by fluorometric experiments where non-fluorescent 1-naphthalenesulfonyl derivatives were synthesized and competitive adsorption experiments were performed. Copyright (C) 1999 Elsevier Science B.V.

本文言語English
ページ(範囲)188-197
ページ数10
ジャーナルBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
1433
1-2
DOI
出版ステータスPublished - 1999 8 17

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学

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