Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression: A Gynaecological Oncology Group Study

S. Y. Liao, W. H. Rodgers, J. Kauderer, K. M. Darcy, R. Carter, N. Susumu, S. Nagao, J. L. Walker, M. Hatae, E. J. Stanbridge

研究成果: Article

7 引用 (Scopus)

抄録

Background: Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. It has been proposed that LEGH may represent a precursor lesion to a group of mucinous adenocarcinoma with gastric phenotype (GA) that is independent of high-risk human papillomavirus (H-HPV) infection. Carbonic anhydrase-IX (CA-IX) is highly expressed in conventional glandular lesions (CGLs). However, expression of CA-IX in LEGH or GA has not been studied. Methods: In all, 12 CGLs, 7 LEGHs, 6 LEGHs with coexisting adenocarcinoma in situ (AIS, 3) and GA (3) were identified from Japanese women with a cytological diagnosis of atypical glandular cells of undetermined significance. Immunostaining was used to detect CA-IX and p16 INK 4 a (hereafter termed p16) protein expression in the tissues and CA-IX protein expression in the Papanicolaou smears (PSs). Polymerase chain reaction was used to detect H-HPV DNA in liquid-based cytology. Results: Out of 12 (83%) CGLs, 10 were positive with H-HPV and high levels of CA-IX expression were seen in all (100%) cases. P16 protein expression was observed in 11 out of 12 (92%) cases. None of the LEGHs, LEGHs with AIS or GA were positive for H-HPV and only 8 out of 13 (62%) showed focal weak (1+) p16 expression. In contrast, all cases (100%) exhibited strong CA-IX protein expression. Conclusion: Our study suggests that there are different molecular mechanisms of carcinogenesis resulting in CGLs vs LEGHs associated with AIS or GA. There is also a possible link between LEGHs and GAs. Furthermore, CA-IX expression may serve as a useful biomarker for the detection of GAs in the absence of H-HPV infection.

元の言語English
ページ(範囲)613-620
ページ数8
ジャーナルBritish Journal of Cancer
108
発行部数3
DOI
出版物ステータスPublished - 2013 2 19

Fingerprint

Hyperplasia
Neoplasms
Papillomavirus Infections
Proteins
Papanicolaou Test
Mucinous Adenocarcinoma
Carbonic Anhydrase IX
Cervix Uteri
Cell Biology
Stomach
Carcinogenesis
Biomarkers
Phenotype
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

これを引用

Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression : A Gynaecological Oncology Group Study. / Liao, S. Y.; Rodgers, W. H.; Kauderer, J.; Darcy, K. M.; Carter, R.; Susumu, N.; Nagao, S.; Walker, J. L.; Hatae, M.; Stanbridge, E. J.

:: British Journal of Cancer, 巻 108, 番号 3, 19.02.2013, p. 613-620.

研究成果: Article

Liao, S. Y. ; Rodgers, W. H. ; Kauderer, J. ; Darcy, K. M. ; Carter, R. ; Susumu, N. ; Nagao, S. ; Walker, J. L. ; Hatae, M. ; Stanbridge, E. J. / Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression : A Gynaecological Oncology Group Study. :: British Journal of Cancer. 2013 ; 巻 108, 番号 3. pp. 613-620.
@article{68fd9350db6941eaa520a83157c57e20,
title = "Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression: A Gynaecological Oncology Group Study",
abstract = "Background: Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. It has been proposed that LEGH may represent a precursor lesion to a group of mucinous adenocarcinoma with gastric phenotype (GA) that is independent of high-risk human papillomavirus (H-HPV) infection. Carbonic anhydrase-IX (CA-IX) is highly expressed in conventional glandular lesions (CGLs). However, expression of CA-IX in LEGH or GA has not been studied. Methods: In all, 12 CGLs, 7 LEGHs, 6 LEGHs with coexisting adenocarcinoma in situ (AIS, 3) and GA (3) were identified from Japanese women with a cytological diagnosis of atypical glandular cells of undetermined significance. Immunostaining was used to detect CA-IX and p16 INK 4 a (hereafter termed p16) protein expression in the tissues and CA-IX protein expression in the Papanicolaou smears (PSs). Polymerase chain reaction was used to detect H-HPV DNA in liquid-based cytology. Results: Out of 12 (83{\%}) CGLs, 10 were positive with H-HPV and high levels of CA-IX expression were seen in all (100{\%}) cases. P16 protein expression was observed in 11 out of 12 (92{\%}) cases. None of the LEGHs, LEGHs with AIS or GA were positive for H-HPV and only 8 out of 13 (62{\%}) showed focal weak (1+) p16 expression. In contrast, all cases (100{\%}) exhibited strong CA-IX protein expression. Conclusion: Our study suggests that there are different molecular mechanisms of carcinogenesis resulting in CGLs vs LEGHs associated with AIS or GA. There is also a possible link between LEGHs and GAs. Furthermore, CA-IX expression may serve as a useful biomarker for the detection of GAs in the absence of H-HPV infection.",
author = "Liao, {S. Y.} and Rodgers, {W. H.} and J. Kauderer and Darcy, {K. M.} and R. Carter and N. Susumu and S. Nagao and Walker, {J. L.} and M. Hatae and Stanbridge, {E. J.}",
year = "2013",
month = "2",
day = "19",
doi = "10.1038/bjc.2012.578",
language = "English",
volume = "108",
pages = "613--620",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Endocervical glandular neoplasia associated with lobular endocervical glandular hyperplasia is HPV-independent and correlates with carbonic anhydrase-IX expression

T2 - A Gynaecological Oncology Group Study

AU - Liao, S. Y.

AU - Rodgers, W. H.

AU - Kauderer, J.

AU - Darcy, K. M.

AU - Carter, R.

AU - Susumu, N.

AU - Nagao, S.

AU - Walker, J. L.

AU - Hatae, M.

AU - Stanbridge, E. J.

PY - 2013/2/19

Y1 - 2013/2/19

N2 - Background: Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. It has been proposed that LEGH may represent a precursor lesion to a group of mucinous adenocarcinoma with gastric phenotype (GA) that is independent of high-risk human papillomavirus (H-HPV) infection. Carbonic anhydrase-IX (CA-IX) is highly expressed in conventional glandular lesions (CGLs). However, expression of CA-IX in LEGH or GA has not been studied. Methods: In all, 12 CGLs, 7 LEGHs, 6 LEGHs with coexisting adenocarcinoma in situ (AIS, 3) and GA (3) were identified from Japanese women with a cytological diagnosis of atypical glandular cells of undetermined significance. Immunostaining was used to detect CA-IX and p16 INK 4 a (hereafter termed p16) protein expression in the tissues and CA-IX protein expression in the Papanicolaou smears (PSs). Polymerase chain reaction was used to detect H-HPV DNA in liquid-based cytology. Results: Out of 12 (83%) CGLs, 10 were positive with H-HPV and high levels of CA-IX expression were seen in all (100%) cases. P16 protein expression was observed in 11 out of 12 (92%) cases. None of the LEGHs, LEGHs with AIS or GA were positive for H-HPV and only 8 out of 13 (62%) showed focal weak (1+) p16 expression. In contrast, all cases (100%) exhibited strong CA-IX protein expression. Conclusion: Our study suggests that there are different molecular mechanisms of carcinogenesis resulting in CGLs vs LEGHs associated with AIS or GA. There is also a possible link between LEGHs and GAs. Furthermore, CA-IX expression may serve as a useful biomarker for the detection of GAs in the absence of H-HPV infection.

AB - Background: Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. It has been proposed that LEGH may represent a precursor lesion to a group of mucinous adenocarcinoma with gastric phenotype (GA) that is independent of high-risk human papillomavirus (H-HPV) infection. Carbonic anhydrase-IX (CA-IX) is highly expressed in conventional glandular lesions (CGLs). However, expression of CA-IX in LEGH or GA has not been studied. Methods: In all, 12 CGLs, 7 LEGHs, 6 LEGHs with coexisting adenocarcinoma in situ (AIS, 3) and GA (3) were identified from Japanese women with a cytological diagnosis of atypical glandular cells of undetermined significance. Immunostaining was used to detect CA-IX and p16 INK 4 a (hereafter termed p16) protein expression in the tissues and CA-IX protein expression in the Papanicolaou smears (PSs). Polymerase chain reaction was used to detect H-HPV DNA in liquid-based cytology. Results: Out of 12 (83%) CGLs, 10 were positive with H-HPV and high levels of CA-IX expression were seen in all (100%) cases. P16 protein expression was observed in 11 out of 12 (92%) cases. None of the LEGHs, LEGHs with AIS or GA were positive for H-HPV and only 8 out of 13 (62%) showed focal weak (1+) p16 expression. In contrast, all cases (100%) exhibited strong CA-IX protein expression. Conclusion: Our study suggests that there are different molecular mechanisms of carcinogenesis resulting in CGLs vs LEGHs associated with AIS or GA. There is also a possible link between LEGHs and GAs. Furthermore, CA-IX expression may serve as a useful biomarker for the detection of GAs in the absence of H-HPV infection.

UR - http://www.scopus.com/inward/record.url?scp=84875229171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875229171&partnerID=8YFLogxK

U2 - 10.1038/bjc.2012.578

DO - 10.1038/bjc.2012.578

M3 - Article

C2 - 23299542

AN - SCOPUS:84875229171

VL - 108

SP - 613

EP - 620

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 3

ER -