Endogenous glutamatergic synaptic activity elicits acetylcholine release from rat cultured septal cells

Takeshi Suzuki, Takeshi Matsugi, Ryoichi Takagi, Koichiro Kawashima

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We tested the characteristics of acetylcholine (ACh) release from cultured rat septal cells. The spontaneous release was inhibited by treatment with tetrodotoxin (TTX) and omega-conotoxin (GVIA), indicating that the release was elicited by synaptic activity. The release was also inhibited by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor blocker, in both the absence and presence of nerve growth factor (NGF), suggesting that endogenously released glutamate produced the ACh release by stimulating AMPA receptors. This is the first report of detection of the release of ACh by endogenous spontaneous synaptic activity conducted by glutamate AMPA receptor activation in cultured septal cells. This in vitro experimental system is useful for the study of cholinergic functions.

本文言語English
ページ(範囲)341-347
ページ数7
ジャーナルNeuroscience Research
47
3
DOI
出版ステータスPublished - 2003 11月 1

ASJC Scopus subject areas

  • 神経科学(全般)

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